David M. Warshaw, Ph.D.


David M.  Warshaw, Ph.D.

Professor and Chair

Background

Dr. Warshaw received his Ph.D. in Physiology and Biophysics at the University of Vermont in 1978, and continued his research studying the molecular mechanism of muscle contraction as a post-doctoral associate at the University of Massachusetts Medical School. He returned to the University of Vermont as an Assistant Professor of Molecular Physiology & Biophysics in 1983 and now is Professor and Chair of the Department. Currently, Dr. Warshaw’s lab is using state-of-the-art single molecule detection and manipulation techniques to characterize the structure and function of myosin molecular motors in normal and disease states of the cardiovascular system.

Contact

Office:
HSRF 116
802-656-4300

Lab:
HSRF 115


Research Description

I received my B.S. in Electrical Engineering from Rutgers University and Ph.D. in Physiology & Biophysics from the University of Vermont. As a postdoc with Fredric Fay at UMass Medical, I studied single smooth muscle cell mechanics. My present research focuses on the structure and function of cardiac muscle contractile proteins as well as non-muscle molecular motors using single molecule biophysical techniques such as laser trapping and super-resolution microscopy. Presently, my lab has two research foci. One area focuses on the molecular mechanism by which myosin binding protein-C modulates cardiac and skeletal muscle contractility, using an in vitro muscle model systems. The other focus is in vitro 3D model systems of intracellular cargo transport by myosin motors. I have been the Principal Investigator of a National Institutes of Health (NIH) Program Project Grant focused on the molecular basis of genetic heart failure. I am an Established Investigator and Fellow of the American Heart Association and a Fellow of the Biophysical Society. I have organized numerous International Conferences and Symposia, including the Gordon Conference on “Muscle Contractile Proteins” (1999, 2002) and was the program co-chair of the 2009 Biophysical Society annual meeting. I have and continue to serve on numerous NIH review panels and was a member of the Scientific Advisory Panel for the NIH Nanomedicine Initiative. I have trained 26 pre- and postdoctoral fellows of which 17 have gone on to university faculty positions.

Current Publications:

Lombardo AT, Nelson SR, Kennedy GG, Trybus KM, Walcott S, Warshaw DM (2019) Myosin Va transport of liposomes in three-dimensional actin networks is modulated by actin filament density, position, and polarity. Proc Natl Acad Sci U S A 116(17): 8326-8335.

Inchingolo AV, Previs SB, Previs MJ, Warshaw DM, Kad NM (2019) Revealing the mechanism of how cardiac myosin-binding protein C N-terminal fragments sensitize thin filaments for myosin binding. Proc Natl Acad Sci U S A 116(14): 6828-6835.

Nelson SR, Kathe SD, Hilzinger TS, Averill AM, Warshaw DM, Wallace SS, Lee AJ (2019) Single molecule glycosylase studies with engineered 8-oxoguanine DNA damage sites show functional defects of a MUTYH polyposis variant. Nucleic Acids Res 47(6): 3058-3071.

Powell CJ, Ramaswamy R, Kelsen A, Hamelin DJ, Warshaw DM, Bosch J, Burke JE, Ward GE, Boulanger MJ (2018) Structural and mechanistic insights into the function of the unconventional class XIV myosin MyoA from Toxoplasma gondii. Proc Natl Acad Sci U S A 115(45): E10548-E10555.

Papadaki M, Holewinski RJ, Previs SB, Martin TG, Stachowski MJ, Li A, Blair CA, Moravec CS, Van Eyk JE, Campbell KS, Warshaw DM, Kirk JA (2018) Diabetes with heart failure increases methylglyoxal modifications in the sarcomere, which inhibit function. JCI Insight 3(20): .

Lin BL, Li A, Mun JY, Previs MJ, Previs SB, Campbell SG, Dos Remedios CG, Tombe PP, Craig R, Warshaw DM, Sadayappan S (2018) Skeletal myosin binding protein-C isoforms regulate thin filament activity in a Ca2+-dependent manner. Sci Rep 8(1): 2604.

Powell CJ, Jenkins ML, Parker ML, Ramaswamy R, Kelsen A, Warshaw DM, Ward GE, Burke JE, Boulanger MJ (2017) Dissecting the molecular assembly of the Toxoplasma gondii MyoA motility complex. J Biol Chem 292(47): 19469-19477.

 

All Warshaw publications