{"id":131,"date":"2012-12-03T10:31:16","date_gmt":"2012-12-03T14:31:16","guid":{"rendered":"http:\/\/blog.uvm.edu\/dwarshaw\/?page_id=131"},"modified":"2017-08-28T19:07:42","modified_gmt":"2017-08-28T19:07:42","slug":"thin-filament-regulation","status":"publish","type":"page","link":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/research-interests\/thin-filament-regulation\/","title":{"rendered":"Thin Filament Activation by MyBP-C"},"content":{"rendered":"<table style=\"width: 900px;\" border=\"0\">\n<tbody>\n<tr>\n<td width=\"30%\"><div id='Research_Interests' class='widgets_on_page wop_tiny1  wop_small1  wop_medium1  wop_large1  wop_wide1'>\n\t\t\t<ul><li id=\"nav_menu-9\" class=\"widget widget_nav_menu\"><div class=\"menu-research-interests-container\"><ul id=\"menu-research-interests\" class=\"menu\"><li id=\"menu-item-407\" class=\"menu-item menu-item-type-custom menu-item-object-custom menu-item-407\"><a href=\"http:\/\/physioweb.uvm.edu\/warshaw-lab\/research-interests\/\">Myosin: A Molecular Motor<\/a><\/li>\n<li id=\"menu-item-403\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-403\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/research-interests\/myosin-binding-protein-c-mybp-c\/\">Myosin Binding Protein-C (MyBP-C)<\/a><\/li>\n<li id=\"menu-item-404\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-404\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/research-interests\/single-molecule-biophysical-techniques\/\">Single Molecule <br>Biophysical Techniques<\/a><\/li>\n<li id=\"menu-item-400\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-400\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/research-interests\/intracellular-cargo-transport\/\">Intracellular Cargo Transport<\/a><\/li>\n<li id=\"menu-item-399\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-399\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/research-interests\/heart-disease-associated-with-cmybp-c-mutations\/\">Heart and Skeletal Muscle Diseases Associated with cMyBP-C Mutations<\/a><\/li>\n<li id=\"menu-item-405\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-405\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/research-interests\/thin-filament-regulation\/\">Thin Filament Activation by cMyBP-C<\/a><\/li>\n<\/ul><\/div><\/li><\/ul><\/div><!-- widgets_on_page --><\/td>\n<td>\n<h4 align=\"left\">RESEARCH INTERESTS<\/h4>\n<h1 align=\"left\">Thin Filament\u00a0Activation by MyBP-C<\/h1>\n<p align=\"left\">Force generation in striated muscle results from myosin cyclically interacting with actin, a process regulated by changes in intracellular calcium and mediated through the actin-associated regulatory proteins, troponin (Tn) and tropomyosin (Tm). Studies suggest that the calcium-dependent movement of Tn-Tm on actin functions as an \u201con-off\u201d switch, regulating myosin binding to actin. To understand how Tn-Tm regulates myosin binding to actin and the potential sensitizing effects of MyBP-C binding to the thin filament, we study the regulatory process at the molecular level using reconstituted single thin filaments in the <em>in vitro<\/em> motility assay. Using a new \u201ctightrope\u201d assay developed by <a href=\"https:\/\/www.kent.ac.uk\/bio\/profiles\/staff\/kad.html\" target=\"_blank\" rel=\"noopener noreferrer\">Dr. Neil Kad<\/a>, our labs characterize the mechanism by which MyBP-C sensitizes the native thin filament to calcium.<\/p>\n<p align=\"left\"><a href=\"http:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-content\/uploads\/sites\/2\/2012\/12\/tightrope_anim.gif\"><img loading=\"lazy\" class=\"aligncenter wp-image-810 size-full\" src=\"http:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-content\/uploads\/sites\/2\/2012\/12\/tightrope_anim.gif\" alt=\"Tightrope\" width=\"184\" height=\"72\" \/><\/a><\/p>\n<p><a href=\"http:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-content\/uploads\/sites\/2\/2012\/12\/Loopedmovie_anim_finalScaled.gif\"><img loading=\"lazy\" class=\"aligncenter wp-image-824 size-full\" src=\"http:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-content\/uploads\/sites\/2\/2012\/12\/Loopedmovie_anim_finalScaled.gif\" alt=\"\" width=\"450\" height=\"218\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>RESEARCH INTERESTS Thin Filament\u00a0Activation by MyBP-C Force generation in striated muscle results from myosin cyclically interacting with actin, a process regulated by changes in intracellular calcium and mediated through the actin-associated regulatory proteins, troponin (Tn) and tropomyosin (Tm). Studies suggest &hellip; <a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/research-interests\/thin-filament-regulation\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":13,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"sidebar-page.php","meta":[],"_links":{"self":[{"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages\/131"}],"collection":[{"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/comments?post=131"}],"version-history":[{"count":12,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages\/131\/revisions"}],"predecessor-version":[{"id":825,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages\/131\/revisions\/825"}],"up":[{"embeddable":true,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages\/13"}],"wp:attachment":[{"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/media?parent=131"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}