{"id":187,"date":"2012-12-10T11:21:15","date_gmt":"2012-12-10T15:21:15","guid":{"rendered":"http:\/\/blog.uvm.edu\/dwarshaw\/?page_id=187"},"modified":"2017-09-20T18:28:50","modified_gmt":"2017-09-20T18:28:50","slug":"in-vitro-motility-assay","status":"publish","type":"page","link":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/techniques\/in-vitro-motility-assay\/","title":{"rendered":"In Vitro Motility Assay"},"content":{"rendered":"<table style=\"width: 900px;\" border=\"0\" cellspacing=\"5\" cellpadding=\"5\">\n<tbody>\n<tr>\n<td width=\"25%\"><div id='Techniques_Menu' class='widgets_on_page wop_tiny1  wop_small1  wop_medium1  wop_large1  wop_wide1'>\n\t\t\t<ul><li id=\"nav_menu-8\" class=\"widget widget_nav_menu\"><div class=\"menu-techniques-container\"><ul id=\"menu-techniques\" class=\"menu\"><li id=\"menu-item-322\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-322\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/techniques\/in-vitro-motility-assay\/\">In Vitro Motility Assay<\/a><\/li>\n<li id=\"menu-item-324\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-324\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/techniques\/laser-trap-assay\/\">Laser Trap Assay<\/a><\/li>\n<li id=\"menu-item-326\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-326\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/techniques\/tirfm-laser-trap-assay\/\">TIRFM \u2013 Laser Trap Assay<\/a><\/li>\n<li id=\"menu-item-800\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-800\"><a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/storm-microscopy\/\">STORM Microscopy<\/a><\/li>\n<\/ul><\/div><\/li><\/ul><\/div><!-- widgets_on_page --><\/td>\n<td width=\"623\">\n<p>This assay represents a simplified model of muscle force and motion generation.\u00a0 In\u00a0this assay, myosin, a molecular motor, is adhered to a nitrocellulose-coated microscope coverslip. The coverslip is one surface of a 20 \u00b5l experimental microchamber. Fluorescently labeled actin filaments in a solution containing MgATP are perfused into the chamber and then the movement of single actin filaments over the myosin surface is visualized in a <strong>T<\/strong>otal <strong>I<\/strong>nternal <strong>R<\/strong>eflectance <strong>F<\/strong>luorescence (TIRF) microscope. Variations of this assay include the use of native myosin thick filaments and native actin thin filaments.<\/p>\n<p><img loading=\"lazy\" class=\"size-full wp-image-459 alignleft\" src=\"http:\/\/physioweb.physiol.w3.uvm.edu\/warshaw-lab\/wp-content\/uploads\/sites\/2\/2012\/12\/InVitroanim.gif\" alt=\"InVitroanim\" width=\"350\" height=\"171\" \/><\/p>\n<p><iframe loading=\"lazy\" width=\"300\" height=\"320\" src=\"https:\/\/streaming.uvm.edu\/media\/embed\/4131\/?r&#038;a\"  frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>This assay represents a simplified model of muscle force and motion generation.\u00a0 In\u00a0this assay, myosin, a molecular motor, is adhered to a nitrocellulose-coated microscope coverslip. The coverslip is one surface of a 20 \u00b5l experimental microchamber. Fluorescently labeled actin filaments &hellip; <a href=\"https:\/\/physioweb.uvm.edu\/warshaw-lab\/techniques\/in-vitro-motility-assay\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":15,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages\/187"}],"collection":[{"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/comments?post=187"}],"version-history":[{"count":20,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages\/187\/revisions"}],"predecessor-version":[{"id":861,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages\/187\/revisions\/861"}],"up":[{"embeddable":true,"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/pages\/15"}],"wp:attachment":[{"href":"https:\/\/physioweb.uvm.edu\/warshaw-lab\/wp-json\/wp\/v2\/media?parent=187"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}