Carolyn’s study of tumor cell dependency on KIF18A is out in Nature Communications

This study describes an unexpected dependency of tumor cells that exhibit chromosome instability (CIN) on KIF18A for proliferation and survival.

The tumor cell screens in this study were greatly facilitated by a collaboration with Joe Clayton at BioTek Instruments. Funding from Susan G. Komen also brought together a fantastic team of scientists, clinicians, and patient advocates that provided key insights for the design of this work.

A press release summarizing the impact of the work and its connection to two recently published Nature papers can be found here.

Congratulations to Carolyn Marquis, who began this work as an undergraduate researcher in the lab, and all of the authors!

Our collaborative work on vulnerabilities of aneuploid cells is out in Nature

This study, led by Uri Ben-David at Tel Aviv University, uncovers genes that aneuploid tumor cells depend on more than near-diploid cells, including spindle assembly checkpoint components and KIF18A. This major collaborative effort involved six labs in five different countries.

Congrats to Stumpff Lab members Carolyn Marquis and Heidi Malaby, who co-authored this paper! A press release describing the work can be found here and a complementary co-published study from Neil Ganem’s lab on genes required for genome duplicated tumor cells can be found here.

Leslie’s studies of micronuclear envelope rupture are now available as a preprint

In collaboration with Laura Reinholdt, Leslie Sepaniac examined the impact and stability of micronuclei formed in vivo due to loss of Kif18a both in normal tissues and in tumors that develop in p53 null mice. She shows that micronuclei in Kif18a loss of function cells have stable nuclear envelopes both in vivo and in vitro and that this is likely explained by the subcellular positioning of lagging chromosomes that form micronuclei in late mitosis. Check out the preprint here.

Preprints describing vulnerabilities of aneuploid/ CIN cells have been posted

Three manuscripts describing possible approaches to specifically inhibit the growth of aneuploid and chromosomally unstable cancer cells were recently posted on bioRxiv. Our lab contributed to two of these studies, one in collaboration with Uri Ben-David at Tel Aviv University, and we were happy to coordinate preprint submission with Neil Ganem and his lab’s work on cells that have undergone whole genome duplication.

Please check out all 3 papers if you’re interested:
https://www.biorxiv.org/content/10.1101/2020.06.18.159327v1
https://www.biorxiv.org/content/10.1101/2020.06.18.159038v1
https://www.biorxiv.org/content/10.1101/2020.06.18.159095v1

Alex Thompson awarded F31 NIH fellowship

Alex Thompson was awarded an F31 NRSA fellowship from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) to investigate the “Molecular etiology of Spondyloepimetaphyseal Dysplasia with joint laxity, leptodactylic type.” Congratulations Alex!

Our study of why chromosomes align during mitosis is out in JCB

In collaboration with Laura Reinholdt at the Jackson Laboratory, we used cell culture and mouse models lacking Kif18a function to investigate the consequences of cell division in the absence of chromosome alignment. These studies suggest that a major function of chromosome alignment is to promote interchromosomal compaction during anaphase and organization of all chromosomes into a single, ovoid nucleus at the completions of cell division. You can find the manuscript in JCB here. Congratulations to Stumpff lab members Cindy Fonseca, Heidi Malaby, Leslie Sepaniac, and Dana Messinger and Reinholdt lab members Whitney Martin, Candice Byers, and Anne Czechanski on a very story!