PREPRINTS
Thompson AF, Blackburn PR, Babovic-Vuksanovic D, Lian JB, Klee EW, and Stumpff J. (2021) Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation. bioRxiv doi: https://www.biorxiv.org/content/10.1101/2021.09.29.462402v2.https://www.biorxiv.org/content/10.1101/2021.09.29.462402v2 (final version published in eLife June 22, 2022)


Solon AL*, Tan Z*, Schutt KL, Jepsen L, Haynes SE, Nesivizhskii AI, Sept D, Stumpff J, Ohi R, and Cianfrocco MA  (2021) Kinesin-binding protein remodels the kinesin motor to prevent microtubule-binding. bioRxiv doi: https://doi.org/10.1101/2021.06.02.446814 (*equal contribution) (Final version published in Sci Adv Nov 19, 2021)
Sepaniac LA, Martin W, Dionne LA, Stearns TM, *Reinholdt LG, and *Stumpff J (2020) Micronuclei arising due to loss of KIF18A form stable micronuclear envelopes and do not promote tumorigenesis. bioRxiv doi: https://doi.org/10.1101/2020.11.23.394924 (*co-corresponding authors) (final version published in JCB  Sept 13th, 2021)
Marquis C, Fonseca CL, Wood L, Malaby HLH, Clayton JE, and Stumpff J. (2020). Chromosomally unstable tumor cells specifically require KIF18A for proliferation. bioRxiv doi: https://doi.org/10.1101/2020.06.18.159327
Cohen-Sharir Y, McFarland JM, Abdusamad M, Marquis C, Tang H, Ippolito MR, Bernhard SV, Laue K, Malaby HLH, Jones A, Kazachkova M, Lyons N, Nagaraja A, Bass AJ, Beroukhim R, Santaguida S, Stumpff J, Golub TR, Storchova Z, and Ben-David U. (2020). Selective vulnerability of aneuploid human cancer cells to inhibition of the spindle assembly checkpoint. bioRxiv doi: https://doi.org/10.1101/2020.06.18.159038 (Final version published in Nature Jan 27, 2021)
Malaby HLH, Lessard DV, Berger CL, and Stumpff J. (2018). Human kinesin Kif18A’s neck linker permits navigation of microtubule bound obstacles within the mitotic spindle. bioRxiv doi: https://doi.org/10.1101/364380 (final version published in Life Science Alliance Jan 17th, 2019)
Fonseca CL, Malaby HLH, Sepaniac LA, Martin W, Byers C, Czechanski A, Messinger D, Tang ME, Ohi R, Reinholdt LG, and Stumpff J. (2018). Mitotic chromosome alignment is required for proper nuclear envelope reassembly. bioRxiv doi: https://doi.org/10.1101/343475, highlighted by preLights (final version published in JCB Feb 7th, 2019)
Malaby H, Dumas M, Ohi R, and Stumpff J. (2018). Kinesin binding protein (KBP) buffers the activity of Kif18A and Kif15 in mitosis to ensure accurate chromosome segregation. bioRxiv doi: https://doi.org/10.1101/343046. (final version published in JCB Feb 1st, 2019)
Kim H and Stumpff J. (2018). Kif18A promotes Hec1 dephosphorylation to coordinate chromosome alignment with kinetochore microtubule attachment. bioRxiv doi: https://doi.org/10.1101/304147.
SELECTED PUBLICATIONS
Thompson AF, Blackburn PR, Babovic-Vuksanovic D, Lian JB, Klee EW, and Stumpff J. Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation.  eLife. 2022 11:e78653 PMID: 35730929

Thompson AF, Vandal S, Stumpff J. Quantifying changes in chromosome position to access chromokinesin activity. In Hinchcliffe EH (Ed.), Mitosis: Methods and Protocols. 2022 New York, NY: Springer Science & Business Media, LLC. PMID: 34972951


Solon AL*, Tan Z*, Schutt KL, Jepsen L, Haynes SE, Nesivizhskii AI, Sept D, Stumpff J, Ohi R, and Cianfrocco MA. Kinesin-binding protein remodels the kinesin motor to prevent microtubule-binding. Science Advances. 2021 7(47). PMID: 34797717 (*equal contribution).


Sepaniac LA, Martin W, Dionne LA, Stearns TM, *Reinholdt LG, and *Stumpff J.   Micronuclei in Kif18a mutant mice form stable micronuclear envelopes and do not promote tumorigenesis. , JCB. 2021 220 (11). PMID:34515734 (*co-corresponding authors) 
Marquis C, Fonseca CL, Queen KA, Wood L, Vandal SE, Malaby HLH, Clayton JE, and Stumpff J. Chromosomally unstable tumor cells specifically require KIF18A for proliferation. Nat Comm. 2021 12: 1213. PMID: 33619254.
Cohen-Sharir Y, McFarland JM, Abdusamad M, Marquis C, Tang H, Ippolito MR, Bernhard SV, Laue K, Malaby HLH, Jones A, Kazachkova M, Lyons N, Nagaraja A, Bass AJ, Beroukhim R, Santaguida S, Stumpff J, Golub TR, Storchova Z, and Ben-David U. Selective vulnerability of aneuploid human cancer cells to inhibition of the spindle assembly checkpoint. Nature 2021, 590: 486-491. PMID: 33505028
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, and Al-Bassam J. The kinesin-5 tail domain directly modulates the mechanochemical cycle of the motor domain for anti-parallel microtubule sliding. eLife. 2020 PMID: 31958056.
Fonseca CL, Malaby HLH, Sepaniac LA, Martin W, Byers C, Czechanski A, Messinger D, Tang ME, Ohi R, Reinholdt LG, and Stumpff J. Mitotic chromosome alignment ensures mitotic fidelity by promoting interchromosomal compaction during anaphase. JCB. 2019, 218:1148-1163. PMID: 30733233
Malaby H, Dumas M, Ohi R, and Stumpff J. Kinesin binding protein ensures accurate chromosome segregation by buffering Kif18A and Kif15. JCB. 2019, 218:1218-1234. PMID: 30709852
Malaby HLH, Lessard DV, Berger CL, and Stumpff J.  KIF18A’s neck linker permits navigation of microtubule-bound obstacles within the mitotic spindle.  LSA. 2019, 2:e201800169. PMID: 30655363
Muretta JM, Reddy BJN, Scarabelli G, Thompson AF, Jariwala S, Major J, Venere M, Rich JN, Willard B, Thomas DD, Stumpff J, Grant BJ, Gross SP, Rosenfeld SS. A posttranslational modification of the mitotic kinesin Eg5 that enhances its mechanochemical coupling and alters its mitotic function. PNAS. 2018, 115: E1779-E1788. PMID: 29432173.
Tracy KM, Tye CE, Ghule PN, Malaby HLH, Stumpff J, Stein LJ, Stein GS, Lian JB. Mitotically-associated lncRNA (MANCR) affects genomic stability and cell division in aggressive breast cancer. Mol Cancer Res. 2018. PMID: 29378907.
Zhou J, Chan J, Lambelé M, Yusufzai T, Stumpff J, Opresko PL, Thali M, Wallace SS. NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis. Cell Reports. 2017, 20: 2044-2056. PMID: 28854357.
Fonseca C and Stumpff J. Quantification of mitotic chromosome alignment. Methods Mol Biol. 2016, 1413: 253-62. PMID: 27193854. PDF_Fonseca_2016
Czechanski A, Kim H, Byers C, Greenstein I, Stumpff J*, Reinholdt LG*. Kif18a Is specifically required for mitotic progression during germ line development. Dev Biol. 2015 June 15, 402(2):253-62 .PMID:25824710. (*corresponding authors) PDF_Czechanski_2015
Malaby, HLH and Stumpff, J. Microtubule recognition: A curvy attraction. Curr Biol. 2014 Oct 24 (20): R998-R1000. PDF_Malaby_2014
Kim H, Fonseca C and Stumpff J. A unique kinesin-8 surface loop provides specificity for chromosome alignment.  Mol Biol Cell. 2014 Nov 25 (21): 3319-3329.  PMID: 25208566. PDF_Kim_2014
Stumpff J, Ghule PN, Shimamura A, Stein JL, Greenblatt M. Spindle microtubule dysfunction and cancer predisposition.  J Cell Physiol. 2014 Dec, 229 (12): 1881-3. doi: 10.1002/jcp.24691. PMID: 24905602 PDF_Stumpff_2014_JCP
Bissonette S, Stumpff J. Quantifying Mitotic Chromosome Dynamics and Positioning.  J Cell Physiol. 2014 Oct 229(10):1301-05. doi: 10.1002/jcp.24634. PMID: 24683081 PDF_Bissonette_2014

(Featured on journal Cover)
Cunniff B, Snider GW, Fredette N, Stumpff J, Hondal RJ, Heintz NH. Resolution of oxidative stress by thioredoxin reductase: Cysteine versus selenocysteine. Redox Biol. 2014 Feb 19;2:475-84. doi: 10.1016/j.redox.2014.01.021. PMID: 24624337 PDF_Cunniff_2014
Cunniff B, Benson K, Stumpff J, Newick K, Held P, Taatjes D, Joseph J, Kalyanaraman B, Heintz NH. Mitochondrial-targeted nitroxides disrupt mitochondrial architecture and inhibit expression of peroxiredoxin 3 and FOXM1 in malignant mesothelioma cells.  J Cell Physiol. 2013 Apr;228(4):835-45. doi: 10.1002/jcp.24232. PMID: 23018647 PDF_Cunniff_2013

(Featured on journal Cover)
Stumpff J. Measuring microtubule thickness: an exercise in cooperativity.  Dev Cell. 2012 Jul 17;23(1):1-2. doi: 10.1016/j.devcel.2012.06.010. PMID: 22814594 PDF_Stumpff_2012_DC2
Stumpff J**, Wagenbach M, Franck A, Asbury CL, Wordeman L** Kif18A and chromokinesins confine centromere movements via microtubule growth suppression and spatial control of kinetochore tension. Dev Cell. 2012 May 15;22(5):1017-29. doi: 10.1016/j.devcel.2012.02.013. PMID: 22595673 PDF_Stumpff_2012_DC1

(** co-corresponding authors) (Featured on journal Cover) (Cited on F1000 Prime)
Stumpff J**, Du Y**, English CA, Maliga Z, Wagenbach M, Asbury CL, Wordeman L, Ohi R. A tethering mechanism controls the processivity and kinetochore-microtubule plus-end enrichment of the kinesin-8 Kif18A.  Mol Cell. 2011 Sep 2;43(5):764-75. doi: 10.1016/j.molcel.2011.07.022. PMID: 21884977 PDF_Stumpff_2011

(** authors contributed equally) (Cited on F1000 Prime)
Mattison CP, Stumpff J, Wordeman L, Winey M. Mip1 associates with both the Mps1 kinase and actin, and is required for cell cortex stability and anaphase spindle positioning.  Cell Cycle. 2011 Mar 1;10(5):783-93. Epub 2011 Mar 1. PMID: 21325884 PDF_Mattison_2011

(Featured on journal Cover)
Wordeman L, Stumpff J. Microtubule length control, a team sport? Dev Cell. 2009 Oct;17(4):437-8. doi: 10.1016/j.devcel.2009.10.002. PMID: 9853556 PDF_Wordeman_2009
Garcia K, Stumpff J, Duncan T, Su TT. Tyrosines in the kinesin-5 head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity.  Curr Biol. 2009 Oct 13;19(19):1670-6. doi: 10.1016/j.cub.2009.08.013. Epub 2009 Oct 1. PMID: 19800237 PDF_Garcia_2009
Stumpff J, von Dassow G, Wagenbach M, Asbury C, Wordeman L. The kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment. Dev Cell. 2008 Feb;14(2):252-62. doi: 10.1016/j.devcel.2007.11.014. PMID: 18267093 PDF_Stumpff_2008_DevCell

(Cited on F1000 Prime)
Stumpff J, Asbury CL. Chromosome bi-orientation: Euclidian euploidy.  Curr Biol. 2008 Jan 22;18(2):R81-3. doi: 10.1016/j.cub.2007.11.036. PMID: 18211848 PDF_Stumpff_2008_CurrBiol
Stumpff J, Cooper J, Domnitz S, Moore AT, Rankin KE, Wagenbach M, Wordeman L. In vitro and in vivo analysis of microtubule-destabilizing kinesins.  Methods Mol Biol. 2007 ;392:37-49. PMID: 17951709
Stumpff J, Wordeman L.  Chromosome congression: the kinesin-8-step path to alignment. Curr Biol. 2007 May 1;17(9):R326-8. Review. PMID: 17470346 PDF_Stumpff_2007
Stumpff J, Kellogg DR, Krohne KA, Su TT.  Drosophila Wee1 interacts with members of the gammaTURC and is required for proper mitotic-spindle morphogenesis and positioning. Curr Biol. 2005 Sep 6;15(17):1525-34. PMID: 16139207 PDF_Stumpff_2005
Stumpff J, Duncan T, Homola E, Campbell SD, Su TT. Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesis. Curr Biol. 2004 Dec 14;14(23):2143-8. PMID: 15589158 PDF_Stumpff_2004

(Cited on F1000 Prime)
Su TT, Walker J, Stumpff J. Activating the DNA damage checkpoint in a developmental context.  Curr Biol. 2000 Feb 10;10(3):119-26. PMID: 10679321 PDF_Su_2000
Full list of Dr. Stumpff's publications on Pubmed